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KMID : 1137020220330040050
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Journal of Gynecologic Oncology
2022 Volume.33 No. 4 p.50 ~ p.50
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TP53 variants in p53 signatures and the clonality of STICs in RRSO samples
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Akahane Tomoko
Masuda Kenta
Hirasawa Akira
Kobayashi Yusuke
Ueki Arisa
Kawaida Miho
Misu Kumiko
Nakamura Kohei
Nagai Shimpei
Chiyoda Tatsuyuki
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Abstract
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Objective: Precursor lesions may be identified in fallopian tube tissue after risk-reducing salpingo-oophorectomy (RRSO) in patients with pathogenic variants of BRCA1/2. Serous tubal intraepithelial carcinoma (STIC) is considered a precursor of high-grade serous carcinoma, whereas the significance of the p53 signature remains unclear. In this study, we investigated the relationship between the p53 signature and the risk of ovarian cancer.
Methods: We analyzed the clinicopathological findings and conducted DNA sequencing for TP53 variants of p53 signatures and STIC lesions isolated using laser capture microdissection in 13 patients with pathogenic variants of BRCA1/2 who underwent RRSO and 17 control patients with the benign gynecologic disease.
Results: TP53 pathogenic variants were detected significantly higher in RRSO group than control (p<0.001). No difference in the frequency of p53 signatures were observed between groups (53.8% vs 29.4%; p=0.17). TP53 sequencing and next-generation sequencing analysis in a patient with STIC and occult cancer revealed 2 TP53 mutations causing different p53 staining for STICs and another TP53 mutation shared between STIC and occult cancer.
Conclusion: The sequence analysis for TP53 revealed 2 types of p53 signatures, one with a risk of progression to STIC and ovarian cancer with pathological variants in TP53 and the other with a low risk of progression without pathological variants in TP53 as seen in control.
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KEYWORD
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Prophylactic Surgical Procedures, Salpingo-Oophorectomy, Genes, p53, Genes, BRCA1, Genes, BRCA2, Carcinoma in Situ, Cystadenocarcinoma, Serous
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